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Gopala Reddy, A.
- Protective Effect of Curcumin Against Imidacloprid-Induced Genotoxicity in Rats
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Authors
Affiliations
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Hyderabad-500030, Telangana, IN
1 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Hyderabad-500030, Telangana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 2 (2015), Pagination: 65-69Abstract
Aim: To study the effect of curcumin in imidacloprid (IMI)-induced genotoxicity by comet and micronucleus assays.
Materials and Methods: Experiments were conducted on adult rats that were randomly divided into six groups of six rats as follows: Group 1: Sham; Group 2: Curcumin (50 mg/kg po); Group 3: IMI (20 mg/kg po); Group 4: IMI (40 mg/kg po); Group 5: IMI (20 mg/kg po) + curcumin (50 mg/kg po); and Group 6: IMI (40 mg/kg po) + curcumin (50 mg/kg po). Micronucleus and comet assays were used to study the DNA damage in all the groups.
Results: There was a significant (P < 0.05) increase in the comet tail length (μm) and the frequency of micronuclei formation in the Group 4 treated with 40 mg/kg IMI when compared to other groups. Curcumin treatment in Group 6 showed a significant (P < 0.05) decrease in the comet tail length, and micronuclei as compared to Group 4 though the decrease was significantly (P < 0.05) low as compared to other groups.
Conclusions: This study revealed that IMI has a tendency to induce the genotoxicity on exposure to 40 mg/kg b.wt. Curcumin, due to its anti-oxidant and anti-mutagenic property, alleviated the genotoxicity induced by IMI.
Keywords
Comet Assay, Curcumin, DNA, Genotoxicity, Imidacloprid.
References
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- Feng S, Kong Z, Wan X, Peng P, Zeng EY. Assessing the genotoxicity of imidacloprid and RH-5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests. Ecotoxicol Environ Saf. 2005; 61:239–46.
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- Protective Effect of Rutin in Comparison to Silymarin Against Induced Hepatotoxicity in Rats
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Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.
Authors
M. Kasi Reddy
1,
A. Gopala Reddy
1,
B. Kala Kumar
1,
D. Madhuri
2,
G. Boobalan
1,
M. Anudeep Reddy
1
Affiliations
1 Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
2 Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
1 Department of Veterinary Pharmacology and Toxicology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
2 Department of Veterinary Pathology, P V Narsimha Rao Telangana Veterinary University, College of Veterinary Science, Rajendranagar, Hyderabad-500030, Telangana, IN
Source
Veterinary World, Vol 10, No 1 (2017), Pagination: 74-80Abstract
Aim: The aim of this study is to evaluate the hepatoprotective effect of rutin (RTN) in comparison to silymarin (SLM) against acetaminophen (APAP)-induced hepatotoxicity in rats.Materials and Methods: Male Wistar albino rats (n=24) of 3 months age were equally divided into four groups. Group 1 served as normal control. Hepatotoxicity was induced in the remaining three groups with administration of 500 mg/kg po APAP from day 1-3. Groups 2, 3, and 4 were subsequently administered orally with distilled water, 25 mg/kg of SLM, and 20 mg/kg of RTN, respectively, for 11 days. The mean body weights and biomarkers of hepatotoxicity were estimated on day 0, 4 (confirmation of toxicity), and 15 (at the end of treatment). Hematological parameters were evaluated on day 4 and 15. Antioxidant profile and adenosine triphosphatases (ATPases) were assessed at the end of the experiment. Liver tissues were subjected to histopathology and transmission electron microscopy after the sacrifice on day 15.
Results: Antioxidant profile, ATPases, and hematological and sero-biochemical parameters were significantly altered, and histopathological changes were noticed in the liver of toxic control group. These changes were reversed in groups 3 and 4 that were administered with SLM and RTN, respectively.
Conclusion: The results of the present investigation enunciated that SLM has potent hepatoprotective activity though the RTN was found superior in restoring the pathological alterations in paracetamol-induced hepatotoxicity in Wistar albino rats.
Keywords
Acetaminophen, Hepatotoxicity, Rutin, Silymarin.Full Text
References
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- Modulation of Oxidative Stress Mediators in Adjuvant Induced Arthritis by Herbal Adaptogen Boswellia serrata
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Authors
Affiliations
1 JRF, ICAR-NDRI, Southern Regional Station, Adugodi, Bengaluru – 560030, Karnataka, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Kanchikacherla - 505326, Telangana, IN
1 JRF, ICAR-NDRI, Southern Regional Station, Adugodi, Bengaluru – 560030, Karnataka, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Kanchikacherla - 505326, Telangana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 25, No 4 (2018), Pagination: 193-197Abstract
A study was carried out to evaluate Boswellia serrata on adjuvant induced oxidative stress in rats. Intradermal administration of Freund’s adjuvant to induce rheumatoid arthritis consequently produced oxidative damage as indicated by an increase in thiobarbituric acid reducing substances, myeloperoxidase, protein carbonyls and a reduction in superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione in tissue homogenates. Oral administration of methanolic extracts ofBoswellia serrata at 500 mg/kg body weight re-established the antioxidant profile. Co-treatment with an anti-inflammatory agent, meloxicam showed an enhanced healing effect. Hence, a significant restorative effect was witnessed with the herbal adaptogen Boswellia serrata.Keywords
Adjuvant Arthritis, Antioxidants, Boswellia serrata, Oxidative Stress.References
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- Study of Cadmium and Chlorpyrifos Induced Changes in Protein and Lipid Profile in Rats
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Authors
Affiliations
1 Department of Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Jagtial District, Telangana – 505326, Telangana, IN
3 Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telengana, IN
1 Department of Veterinary Pathology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telangana, IN
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science, Korutla, Jagtial District, Telangana – 505326, Telangana, IN
3 Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar, Hyderabad – 500030, Telengana, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 27, No 1&2 (2020), Pagination: 54-57Abstract
The present work was aimed to know the changes in protein and lipid profile induced cadmium and chlorpyrifos Wistar rats. Work was carried out for 28 days in rats. 1: Control. 2: CdCl2 @ 22.5 mg/kg b.wt/oral. 3: CPF @ 25 mg/kg b.wt/per oral. 4: CdCl2@22.5 mg + CPF @ 25 mg/kg b.wt/per oral. Results revealed a significant (P<0.05) decrease in the concentration of total protein and albumin in Groups 2, 3 and 4 and also a significant (P<0.05) increase in total cholesterol, triglycerides and phospholipids in Groups 2, 3 and 4 on 15th and 29th day of the experiment. The adverse changes in protein and lipid profile in combined Group were severe than individual Groups due to synergistic action of the combined pollutants.Keywords
Cadmium, Chlorpyrifos, Lipid Profile, Protein Profile, Wistar Rats.References
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